| Heat Number - 1 | |||||
| Lane | Comp.No. | Name | A.G. | Club/Country | Time |
| 2 | 2 | Aquae Sulis Performanc | Aquae Sulis | 4:06.00 | |
| 3 | 4 | City of Bristol SC | Co Bristol | 3:49.50 | |
| 4 | 6 | University of Bath SC | Bath Univ | 3:45.99 | |
| 5 | 5 | Plymouth Leander SC | Plymouth Lea | 3:47.33 | |
| 6 | 3 | Poole SC | Poole | 3:50.09 | |
| 7 | 1 | Soundwell SC | Soundwell | 4:13.00 | |